Principle of Point Counting Techniques
In the introduction it was argued that the area of
a feature in an area of interest (micrograph or polished surface) could be
estimated by counting the number of squares in a regular grid that lie
predominantly in the feature. To put this concept into efficient practice
requires a simpler approach. It would be easier if each square were marked
at its centre:
Now counting the squares predominantly within the features of interest
can be recast as counting the number of dots at the centre of the squares
that lie in the features of interest. This is obviously much easier, as
no qualitative decision has to be made about whether a square is
predominantly in or out. The dots are mostly going to be in or out.
Part of the trick is to ensure the dots are small enough in relation to the
boundary that dots "on the line" are a rare event.
Of course, having decided that counting points is easier than counting
squares, we can drop the squares!
Now we merely count the points hitting the features of interest. Their
area can be approximated by this number multiplied by the area of a square.
This is the origin of the terminology Point Counting Morphometry.
In the practice of point counting the points
used can take many forms, including grids!
©D.F. Davey,
Department of Physiology,
University of Sydney
Last updated 10 April 2002
Feedback