The mechanisms by which ion transport systems are controlled in mouse early embryos
EA Harding, A Widin, DI Cook, ML Day

In 1997 we completed 3 projects in this area. First, we showed that a large-conductance K⁺ channel, that we have previously shown undergoes cyclic changes in activity in response to the cell-cycle, is controlled by a cell cycle clock that is located in the cell cytoplasm and is independent of the well-known nuclear cell cycle clock. We further showed that this cytosolic clock interacts with the nuclear cell cycle clock and have started to define the basis for these interactions (see diagram below).

Second, we also established that T-type Ca²⁺ channels are controlled by the same cytosolic cell cycle clock. Finally, we investigated the changes in the activity of H⁺ transport systems that occur during pre-implantation development of the mouse.

Development of replication-deficient adenoviruses as a tool in studying the role of membrane